Recombinant RTN4RL2 anticorps (AA 279-420)

N° du produit ABIN5668205

Cet anticorps Lapin Monoclonal détecte spécifiquement RTN4RL2 dans WB, ELISA, IF, FACS et IP. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Recombinant RTN4RL2 anticorps (AA 279-420) (ABIN5668205)

Antigène: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp RTN4RL2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: P14

Détail du produit anti-RTN4RL2 anticorps

Épitope: AA 279-420

Fonction: Anti-NgR2 [P14], Rabbit IgG, kappa

Specificité: The antibody binds to human NgR2 with a Kd of 2.3 nM and to rat NgR2 with a Kd of 0.48 nM (bivalent binding). The antibody binds specifically to residues 351-399 of NgR2, a member of the Nogo-66 receptor family which inhibits axonal growth and sporulating in the CNS.

Réactivité croisée: Humain, Rat

Attributs du produit:

Original Species of Ab: Rabbit

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogène: (His)6-tagged fragment of rat NgR2 (amino acids 279-420).

Isotype: IgG kappa

Information d'application

Indications d'application: The original antibody was used for for an ELISA on the NgR2 antigen to confirm the binding of the antibody. This antibody was used for a western blot on lysates of 293F cells transfected with vectors for mammalian cell expression of human NgR1, NgR2, and NgR3. This antibody was used for flow cytometry on 293F cells transfected with human NgR1, NgR2, and NgR3 mammalian cell expression vectors. This antibody was used for surface plasmon resonance on NgR1 and NgR2 to determine its binding affinity for its antigen. The antibody was used for immunofluorescence on PC12 cells and PC12-NgR1 or PC12-NgR2. This antibody was used for immunoprecipitation on rat PC12 cell lines stably expressing full-length rat NgR1 or NgR2 (Hofer et al., 2008, PMID:17140598).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur RTN4RL2

Antigène: RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))

Autre désignation: NgR2

Sujet: Nogo-66 Receptor

UniProt: Q86UN3